Developing top-quality standard pays to for marketing the quality of traditional Chinese medication shots, which could be examined by establishing the extensive quality control method. A way for multiple determination of salvianolic acid B, rosmarinic acid and lithospermic acid in Salvianolate for Injection was developed for quantitative analysis of multi-components with single-marker(QAMS). ZORBAX Eclipse Plus C_(18) chromatographic column had been followed, with 0.1% phosphoric acid and acetonitrile as mobile phase. The circulation rate ended up being set at 1 mL·min~(-1). The column heat had been set at 20 ℃, while the recognition wavelength had been 286 nm. Salvianolic acid B was utilized as interior guide. The general correction factors of rosmarinic acid and lithospermic acid(f_(s/i)) had been 0.58 and 0.94, respectively. About 85% of substances in Salvianolate for Injection had been quantified because of the founded QAMS method. The evaluation various batches of intermediates and products during four years indicated that the articles of salvianolic acid B had been 77.1%-81.5% in intermediates and 70.5%-80.1% in products; the sum total content of rosmarinic acid and lithospermic acid was about 6%. The ratio of rosmarinic acid to lithospermic acid was(3.4∶1-10∶1) and(2.5∶1-5∶1), respectively, which showed that the ratio was more stable in preparation. The QAMS technique founded is feasible for extensive quality control of multiple components of in Salvianolate for Injection.The conventional Chinese medicine(TCM) quality continual herbal slices assessment strategy ended up being applied to evaluate the grade of Sophorae Tonkinensis Radix et Rhizoma based on the combination of standard personality recognition and modern clinical and technical techniques. The TCM high quality continual evaluation method was used to look for the appearance and index content of medicinal pieces, determine the quality constant and percentile high quality constant of Sophorae Tonkinensis Radix et Rhizoma from various sources, and talk about their classification. The quality constants of 15 batches of Sophorae Tonkinensis Radix et Rhizoma pieces had been between 0.004 and 0.063. The pieces using the percentage quality constant ≥80% were classified as the very first level; people that have the portion quality constant RRx-001 datasheet ≥50% and less then 80% were classified as the 2nd grade; and people with the percentage quality continual less then 50% were categorized since the 3rd quality. Then the pieces with the high quality continual ≥0.050 were categorized once the very first grade; those with the high quality continual ≥0.032 and less then 0.050 were classified given that 2nd quality; and people with all the high quality constant less then 0.032 were categorized because the 3rd grade. In accordance with the outcomes, the 15 batches of Sophorae Tonkinensis Radix et Rhizoma pieces were split into 1 group associated with the first quality, 4 batches associated with second quality, and 10 batches of this 3rd level. The standard continual evaluation method established is medical, unbiased, simple and possible. The effective use of the strategy in Sophorae Tonkinensis Radix et Rhizoma pieces has reasonable outcomes, which can be useful to market the classification of Sophorae Tonkinensis Radix et Rhizoma and promotes the top-notch application of Sophorae Tonkinensis Radix et Rhizoma.To prepare ginkgolide B nanosuspension(GB-NS), and explore its dissolution habits in vitro. The miniaturized news milling strategy had been made use of to prepare nanosuspensions, with average particle size and polydispersity list given that evaluation indexes. The formula and process of GB-NS were optimized by single factor experiment and Box-Behnken design-response area method. The morphology was seen by checking electron microscope(SEM), and thecrystallinity of GB-NS was examined by X-rays diffraction(XRD). The paddle technique ended up being used to review the dissolution of GB-NS in vitro. The mean particle size of optimized GB-NS was(180±7) nm, with a polydispersity list of 0.196±0.036. SEM revealed that GB-NS was rod-like or irregular granular. XRD indicated that the crystallinity of GB-NS had been somewhat paid down in contrast to GB natural material. The cumulative dissolution rate of GB-NS reached 90% in 30 min, which was higher than that of GB natural product. The results advised that the miniaturized media milling method was quick, efficient and feasible to get ready GB-NS. While the dissolution rate of GB ended up being somewhat improved by nanosuspension technology.To enrich the transcriptome data in rhizome of Polygonatum cyrtonema seedlings, recognize candidate functional genetics involved with steroidal saponin biosynthesis and provide hereditary sources for the analysis on anabolism path and regulatory device of active components in P. cyrtonema, Illumina system was applied to execute transcriptomic sequencing of rhizome of P. cyrtonema, accompanied by a few bioinformatics analysis on RNA-seq data, including de novo construction, annotation, classification and metabolic path evaluation for the put together unigene. Meanwhile, a deep analysis regarding the steroidal saponin biosynthesis in secondary kcalorie burning pathway was performed.
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